Alkaloid and benzopyran compounds of Melicope latifolia fruit exhibit anti-hepatitis C virus activities

Abstract Background New agents for developing alternative or complementary medicine to treat the hepatitis C virus (HCV) are still needed due high rates of HCV infection globally and current limitations available treatments. Treatment with a combination direct acting antivirals have been shown be approximately 90% effective but will limited in future emergence drug resistance cost. The leaves Melicope latifolia previously reported anti-HCV activity potential source bioactive compounds novel development. This study aimed evaluate efficacy extract M. fruit isolate its active compounds. Method was extracted using methanol purified vacuum liquid chromatography (VLC) Radial Chromatography. analyzed cell culture lines Huh7it-1 JFH1 (genotype 2a). Time-of-addition immunoblotting studies were performed identify mode action isolated structures determined nuclear magnetic resonance (NMR) spectra, UV, IR, Mass Spectra. Results Six known from fruit: O-methyloktadrenolon, alloevodionol, isopimpinellin, alloxanthoxyletin, methylevodionol, N-methylflindersine. N-methylflidersine most compound IC 50 value 3.8 ?g/ml while alloevodionol less active. O-methyloktadrenolon alloxanthoxyletin moderately values 10.9 21.72 ?g/ml, respectively. decreased level NS3 protein expression cells. Conclusion alkaloid compound, N-methylflindersine which possesses through post-entry inhibition suppressed expression.